Molecular Analysis of the Microorganisms in a Thermophilic CSTR used for Continuous Biohydrogen Production
2005
Oh, Y.K. (Pusan National University, Busan, Republic of Korea) | Park, S.H. (Pusan National University, Busan, Republic of Korea) | Ahn, Y.H. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: yahn@kaist.ac.kr
Molecular methods were employed to investigate microorganisms in a thermophilic continuous stirred tank reactor (CSTR) used for continuous H₂ production. The reactor was inoculated with heat-treated anaerobic sludge and fed with a glucose-based medium. Denaturing gradient gel electrophoresis showed dynamic changes of bacterial populations in the reactor during 43 days of operation. Gas composition was constant from approximately 14 days but population shift still occurred.
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