[Condition of oocyte - cumuli complexes of culled cows and their in vitro fertilization ability]
2008
Letkevitch, L.L. | Gandzha, A.I. | Kostikova, I.V. | Rakovitch, E.D., National Academy of Sciences. Scientific and Practical Center of Animal Breeding (Belarus)
Condition of oocyte - cumuli complexes extracted from ovaries of culled highly productive cows and their correlation with morphofunctional peculiarities of ovaries and ability of in vitro fertilization was studied. It is stated that ovaries at follicular and lutein stage of sex cycle as well as with fresh ovulation contain 60,0-54,4% oocyte - cumuli complexes good for cultivation in vitro that let us get 50,9-24,0% of splitting cells and 15,5% of morul-blastocysts. The same ovaries possess the greatest potentiality for meiosis initiation. Metaphase II is correspondingly reached by 62,2, 50,0 and 60,0% of oocytes. On average 47,1% of oocytes reach metaphase II stage in 24 hours of in vitro cultivation. The greatest number of degenerated oocytes were ob¬served in ovaries of in - calf cows and gonads with hippofunction - 47,8 and 30% correspondingly
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