Construction of a yeast strain with regulatable phospholipid synthesis for analysis of the uptake and metabolism of phosphatidylethanolamine with short acyl chains
2007
Deng, L.(Tokyo Univ. (Japan)) | Kakihara, T. | Fukuda, R. | Ohta, A.
We constructed a yeast stain, TKY12Ga, in which phosphatidylethanolamine (PE) synthesis can be controlled by means of the carbon source in the medium. When PE synthesis was blocked, its growth was inhibited. However, in the presence of exogenous didecanoyl PE (diC10PE), TKY12Ga grew despite an inability to synthesize PE. Our system, which employs TKY12Ga strain and diC10PE, provides a valuable tool to study the transport and metabolism of PE in yeast.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por The Agriculture, Forestry and Fisheries Research Information Technology Center