Construction of a yeast strain with regulatable phospholipid synthesis for analysis of the uptake and metabolism of phosphatidylethanolamine with short acyl chains
2007
Deng, L.(Tokyo Univ. (Japan)) | Kakihara, T. | Fukuda, R. | Ohta, A.
We constructed a yeast stain, TKY12Ga, in which phosphatidylethanolamine (PE) synthesis can be controlled by means of the carbon source in the medium. When PE synthesis was blocked, its growth was inhibited. However, in the presence of exogenous didecanoyl PE (diC10PE), TKY12Ga grew despite an inability to synthesize PE. Our system, which employs TKY12Ga strain and diC10PE, provides a valuable tool to study the transport and metabolism of PE in yeast.
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书目信息
页码
pp. 2313-2315
其它主题
Metabolismo de fosfolipidos; Transfert de gene; Fosfolipidos; Metabolisme des phospholipides
语言
英语
注释
Summary (En)
1 tab. 2 fig. 12 ref.
类型
Summary
2008-05-15
AGRIS AP