Study on Effects of Media, EGF, β-ME and Hormones on IVM of Porcine Oocytes
2006
Jang, S.H. (Chungnam National University, Daejeon, Republic of Korea) | Rhee, M.H. (Kyungpook National University, Daegu, Republic of Korea) | Kim, S.K. (Chungnam National University, Daejeon, Republic of Korea), E-mail: kskkim@cnu.ac.kr
This study was investigated in vitro maturation rate of oocytes cultured in maturation media with or without supplementation of EGF, β-ME and hormones. 1. When the oocytes were culture in TCM-199, NCSU-23 and PZM-3 for 48 hrs, the maturation rates were 25.4±0.70%, 30.4±0.70% and 30.4±2.82%, respectively. The maturation rate of oocytes cultured in TCM-199 was slightly lower than cultured in NCSU-23 and PZM-3 (p less than 0.05). 2. the IVM rate of oocytes cultured in TCM-199, NCSU-23 and PZM-3 supplementation with 25 ng/ml EGF for 48 hrs were 46.3±2.8%, 76.6±3.1% and 72.2±2.6%, respectively.
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