Enhanced Expression of High-affinity Iron Transporters via H-ferritin Production in Yeast
2007
Kim, K.S. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: sukkim@chonbuk.ac.kr | Chang, Y.J. (Chonbuk National University, Jeonju, Republic of Korea) | Chung, Y.J. (Chonbuk National University, Jeonju, Republic of Korea) | Park, C.U. (Chonbuk National University, Jeonju, Republic of Korea) | Seo, H.Y. (Chonbuk National University, Jeonju, Republic of Korea)
Our heterologous expression system of the human ferritin H-chain gene (hfH) allowed us to characterize the cellular effects of ferritin in yeasts. The recombinant Saccharomyces cerevisiae (YGH2) evidenced impaired growth as compared to the control, which was correlated with ferritin expression and with the formation of core minerals. Growth was recovered via the administration of iron supplements. The modification of cellular iron metabolism, which involved the increased expression of high-affinity iron transport genes (FET3 and FTR1), was detected via Northern blot analysis.
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