Survival Rate, Developmental Competence and Sex Ratio of Post-thawed Hanwoo Embryo Following Biopsy
2007
Cho, S.R. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Choi, S.H. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Kim, H.J. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Choe, C.Y. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Jin, H.J. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Cho, C.Y. (National Livestock Research Institute, RDA, Namwon, Republic of Korea) | Son, D.S. (National Livestock Research Institute, RDA, Namwon, Republic of Korea), E-mail: sonds@rda.go.kr
The present study was to conducted the sexing efficiency and accuracy of bovine embryo by LAMP (Loop-mediated isothermal amplification) method, the development of the biopsied embryos into re- reformation and the freezability of these blastocysts by slow-freezing and vitrification. In vivo embryos were superovaluted with gonadotropin (Antorin R-10∨®) for 4 days combined with progestrone releasing intravaginal (CIDR∨®) insertion in Hanwoo donors, and in vitro embryos were used blastocyst embryos at Day 7 or Day 8 after post-insemination in vitro.
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