Evaluation and modification of alkaline lysis plasmid preparation method from Lactobacillus spp.
2007
Lee, D.Y. (Seoul National University, Seoul, Republic of Korea) | Seo, Y.S. (Seoul National University, Seoul, Republic of Korea) | Kang, S.G. (Seoul National University, Seoul, Republic of Korea) | Yoo, H.S. (Seoul National University, Seoul, Republic of Korea), E-mail: yoohs@snu.ac.kr
Lactic acid bacteria (LAB) has been regarded as a useful microorganism and tried to manipulate plasmid DNA for increasing the usefulness. Although several methods have been developed is isolate plasmid DNA from Escherichia coli (E. coli), these methods were not sufficient to apply to LAB with exception of O'Sullivan's lysis method. So, we evaluated plasmid DNA extraction from LAB using general E. coli preparation methods and tried to improve the extraction yield and DNA purity by modifying O'Sullivan's alkaline lysis method.
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