Beagle dogs parentage testing by using 22 ISAG microsatellite markers
2007
Ji, H.J. (Cheju National University, Jeju, Republic of Korea) | Kim, E.H. (Cheju National University, Jeju, Republic of Korea) | Lee, K.K. (Cheju National University, Jeju, Republic of Korea) | Kang, T.Y. (Cheju National University, Jeju, Republic of Korea) | Lee, J.M. (Cheju National University, Jeju, Republic of Korea) | Shin, H.D. (SNP Genetics, Inc., Seoul, Republic of Korea) | Kim, L.H. (SNP Genetics, Inc., Seoul, Republic of Korea) | Yun, Y.M. (Cheju National University, Jeju, Republic of Korea), E-mail: dvmyun@cheju.ac.kr
The objective of the study was to establish routine parentage testing system in Beagle dogs using 22 ISAG (International Society for Animal Genetics) canine microsatellite markers (2005). Blood collections were obtained from a mother dog, 4 candidate father dogs and 3 offspring (n = 8). Genomic DNA samples were extracted from 8 Beagle dogs blood for PCR analysis. PCR products for the allele were analyzed by ABI 3130 DNA Sequencer and GeneScan (Ver 3.0) analysis and Genotyper (Ver. 2.1) software.
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