In vitro propagation of Gladiolus anatolicus (Boiss.) Stapf
2007
Emek, Y. (Adnan Menderes Univ. Aydin (Turkey). Dept. of Biology) | Erdag, B.
An In vitro propagation method was developed for Gladiolus anatolicus (Boiss.) Stapf (Iridaceae) using corm sections. Calli were obtained from longitudinally shaped corm sections in Murashige & Skoog medium supplemented with different concentrations of NAA and without growth regulators. The highest rate of callus formation (75 %) occurred in MS medium containing 8.5mg L-1 NAA. Eight weeks after the culture was initiated, calli in 1cm diameter were taken into regeneration experiments. In these experiments, shoot regeneration was promoted using NAA, BA only, and their combinations. The highest number of shoots per explant was obtained in MS medium containing 0.2 mg L-1 BA and 2 mg L-1 NAA (4.7 shoots per explant). Corm formation in the base of shoots were observed in the medium with 0.1 mg L-1 BA. Additionally, 5-6 cormel per shoot occurred during subculturing. Rooting was obtained in the same medium, but rooting rates in shoots were very low (% 20).
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