Comparative Evaluation of Animal Models for Atopic Dermatitis in NC/Nga Mice
2008
Yun, J.W. (AmorePacific RnD Center, Yongin, Republic of Korea) | Kim, H. (AmorePacific RnD Center, Yongin, Republic of Korea) | Kang, H.J. (AmorePacific RnD Center, Yongin, Republic of Korea) | Koh, J.Y. (AmorePacific RnD Center, Yongin, Republic of Korea) | Kim, B.H. (Keimyung University, Daegu, Republic of Korea), E-mail: amorevet@naver.com
Atopic dermatitis is a chronic and relapsing inflammatory skin disease accompanied by severe itching. Recently, animal models of atopic dermatitis, including NC/Nga mouse and hapten-induced mouse model, have received increasing attention. This study was designed to compare four mouse models for atopic dermatitis: 2,4-dinitrochlorobenzene (DNCB) short-term model, DNCB long-term model, dermatophagoides farina (Df) model, and dermatophagoides pteronyssinus (Dp) model. Repeated application of DNCB, Df, and Dp induced chronic skin inflammation, including thickening of the epidermis with marked hyperkeratosis and parakeratosis, and elevated the serum IgE levels compared than normal mice. Therefore, we suggested that 4 models induce not only AD-like skin lesions but also Th2-dominated immune responses in NC/Nga mice maintained under SPF conditions. Application of DNCB also evoked the increase in number of hindlimb scratching in short-term and long-term model. However, in both Df model and Dp model, there was no significant difference in number of hindlimb scratching between Dp- or Df-treated mice and control mice. The scratching frequency and serum immunoglobulin E (IgE) level in DNCB long-term model are higher than those of DNCB short-term model. Moreover, in DNCB long-term model, an increase in degranulating cutaneous mast cells, together with an increase in serum histamine level, was observed in DNCB-treated mice. And, interleukin-4 (IL-4) in the splenic T cell culture supernatants significantly increased in DNCB-treated mice. Consequently, considering all results, NC/Nga mice model developed through repeated long-term treatment of DNCB may be a good model for studying atopic dermatitis.
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