Microbial production of alkaline proteases by locally isolated Bacillus subtilis PCSIR-5

Nadeem, M.; Baig, S.; Qurat-ul-Ain, S.; Qazi, J.I. . Dept. of Zoology

Microbial production of alkaline proteases by locally isolated Bacillus subtilis PCSIR-5

2006

Nadeem, M. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan)) | Baig, S. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan)) | Qurat-ul-Ain, S. (Pakistan Council of Scientific and Industrial Research Labs. Complex, Lahore (Pakistan)) | Qazi, J.I. (Punjab Univ., Lahore (Pakistan). Dept. of Zoology)

Extra-cellular proteases producing bacteria were isolated from local habitats and screened on 1 skim milk agar plates. Depending upon the zone of hydrolysis strain, 5 were selected for further investigations. Studies regarding the effects of pH, size and age of inoculum revealed that maximum proteolytic activity (107 PU/ml) was indicated after 48 hours by using 10% v/v of 24 h old inoculum in fermentation medium. The enzyme had an optimal pH of 8.5 and exhibited its highest activity at 50 degree C the proteases were then tested for collagenolytic activity on leather dust. Results indicate that protease produced by Bacillus subtilis PCSIR-5 has greater collagenolytic activity and it can be used for bio-treatment of leather dust for reduction of the environmental pollution.

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