Genetic collection and development of near-isogenic lines in durum wheat
2008
Watanabe, N., Ibaraki University.College of Agriculture (Japan)
Genetic collections of tetraploid and hexaploid wheats were utilized to develop near-isogenic lines in durum wheat. The genes to be introduced were located on the specific chromosome and mapped to linkage maps using aneuploid stocks of LD222 and Langdon, Landgdon D-genome chromosome substitution lines, and microsatellite markers. We contributed mapping of the genes for long glumes on chromosomes 7AL and 7BL, genes for brittle rachis on chromosomes 3AS and 3BS and the gene for ligulelessness on chromosome 2BL. The mutant gene for sphaerococcoid grain, S16219, was allelic to S2, which was located on the centromeric region of chromosome 3B in hexaploid wheat. The gene for compact spike, C17648 was located on the chromosome 5AL. Near-isogenic lines were developed for the GA-sensitive Rht genes, Rht14, Rht16 and Rht18, on chromosome 6AS. The multiple alleles at the Rht-B1 locus were introduced to cv. LD222. Triticum polonicum IC 12196 may be considered as new source of reduced height genes. Forty-one near-isogenic lines were developed. Twenty-nine donors of genes were from tetraploid wheat collection for near-isogenic lines, and 12 were from hexaploid wheat accessions for near-isogenic lines. The effort to develop near-isogenic lines was extended to introduce taxonomy-related traits such as spelt and awn on the glumes
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