Analysis of genetic diversity and relationships of wild Guizotia species from Ethiopia using ISSR markers.
2008
Yohannes petros;Merker,Arnulf;Habtamu Zeleke(student)
Genetic relationships and diversity of 45 Guizortia populations eah consisting of ten individuals and belonging to five taxa of the genus Guizoita were analyzed using Inter simple sequence repeat (ISSR) markers.Five ISSR primers generated a total of 145 scorable bands across the 450 individuals used for the study.The percent polymorphic loci for the taxa ranged from 68.2 (G. arborescens) to 88% (G. scabra ssp. schimperi), with G. scabra ssp. scabra, G. zavattarii and G. villosa following G. scabra ssp. schimperi in this order with respect to the abundance of percent polymorphic loci. The shannon-weaver diversity indices (H'), for the five taxa also followed a similar patttern, with G. scabra ssp. schimperi exhibiting the highest H' for G. scabra ssp. scabra, G. villosa and G. zavattarii were 0.7373, 0.6620 and 0.6564, respectively. The least genetic distance (0.1188) was observed between G. scabra ssp. schimperi and G. villosa, revealing closer genetic relationships of the two species with each other than with the others, and the highest genetic distance (0.2740) was observed between G. scabra ssp. schimperi and G. zavattarii.
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