The Effects of Environmental Factors on Biodegradability Test for Lubricant Products
2008
Cho, E.H. (Hoseo University, Asan, Republic of Korea) | Park, K.H. (Hoseo University, Asan, Republic of Korea) | Han, S.W. (The University of Seoul, Seoul, Republic of Korea) | Kim, E.Y. (The University of Seoul, Seoul, Republic of Korea) | Ryu, J.S. (Korea Testing and Research Institute, Seoul, Republic of Korea) | Jang, S.B. (Korea Testing and Research Institute, Seoul, Republic of Korea) | Lee, U.G. (Korea Testing and Research Institute, Seoul, Republic of Korea) | Chae, H.J. (Hoseo University, Asan, Republic of Korea)
Various methods (OECD 301B, ISO 9439 and ASTM 5864) for biodegradability test of lubricants were reviewed, and a standard procedure was developed. Most lubrication products are released in rivers or sea then is degraded by microbial action in aerobic condition. Most international method are based on CO₂ evolution test. Inoculum obtained from a sewage disposal plant and test compound are cultivated in a mineral medium. Organic carbon of the test compound is degraded and oxidized through the enzymatic actions of inoculum, and ultimately mineralized to carbon dioxide. Biodegradability test conditions of lubricant oils were optimized. The highest biodegradability was achieved when the same medium as in ASTM 5864 and inoculum concentration of 10⁴~10∨5 cell/L were used. The optimum standard materials were selected as aniline and sodium acetate. Additionally the effects of inoculum type on microbial growth and biodegradability were examined. Finally the standard operating procedure (SOP) for biodegradability test method was proposed.
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