Determination of eugenol in Eugenia caryophyllata by high-performance liquid chromatography with photodiode array detection and method validation
2008
Yun, H.J. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Yun, S.M. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea) | Lee, M.H. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea), E-mail: leemh@nvrqs.go.kr | Son, S.W. (National Veterinary Research and Quarantine Service, Anyang, Republic of Korea)
A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at 60℃ for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients(r² greater then 0.9999) from 0.0125~1 ㎍/ml. The limit of detection per sample injection (20 ㎕) was 0.81 ng/㎕ and limit of quantification was 2.47 ng/㎕. The method showed good intra-day precision (%RSD 0.08~0.27%) and inter-day precision (%RSD 0.32~1.19%).
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