Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains
2009
Zhang, C.X., Chinese Academy of Sciences, Beijing, China | Zhao, X., Chinese Academy of Sciences, Beijing, China | Han, F., Chinese Academy of Sciences, Beijing, China | Yang, M.F., Chinese Academy of Sciences, Beijing, China | Chen, H., Chinese Academy of Sciences, Beijing, China | Chida, T., Chemicals Agrochemicals Planning and Development Department, Tokyo, Japan | Shen, S.H., Chinese Academy of Sciences, Beijing, China
Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class Ⅲ stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.
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