Differential expression of soybean SLTI100 gene encoding translation elongation factor 1A by abiotic stresses
2009
Chung, E.S., Dong-A University, Busan, Republic of Korea | Cho, C.W., Dong-A University, Busan, Republic of Korea | So, H.A., Dong-A University, Busan, Republic of Korea | Yun, B.H., Korea University, Seoul, Republic of Korea | Lee, J.H., Dong-A University, Busan, Republic of Korea
The translation elongation factor 1A, eEF1A, catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome by a GTP-dependent mechanism. By subtractive suppression hybridization technique, we have isolated a soybean low-temperature inducible gene, SLTI100 encoding translation elongation factor 1A. Multiple sequence alignments and phylogenic analysis showed that SLTI100 and other eEF1As originated from diverse organisms are highly conserved. RNA expression of SLTI100 was specifically induced by low temperature, high salt, ABA, or drought stress. Based on the subcellular localization of the corresponding gene product fused to GFP, we were able to confirm that SLTI100-GFP was restricted to the nucleus and cytoplasm. We propose that soybean eEF1A may play an important role in translational regulation during abiotic stress responses in plants.
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