Vegetative incompatibility of isolates of Phomopsis spp. from plum trees | Vegetativna inkompatibilnost izolata Phomopsis spp. izolovanih sa stabla sljive
2008
Zivkovic, S., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Stojanovic, S., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Gavrilovic, V., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Ivanovic, Z., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia)
Isolates of Phomopsis spp. were obtained from plum trees (Prunus domestica L.) with symptoms of die-back. There were significant morpho-physiological and pathogenic differences between the isolates. The genetic variability (12 isolates of Phomopsis spp. and isolate of Phomopsis perniciosa, which was used as control) was proved by study of vegetative compatibility. These isolates were inoculated in Petri dishes in pairs in all possible cross combinations, on potato dextrose agar. After 5 and 10 days the interaction between colonies were scored according to appearance of contact lines of paired cultures. On the contact line on two different isolates distinct barrage zones of inhibition - vegetative incompatibility reaction. The barrage zones generally became black and sometimes showed formation of pycnidia. Necrotic parts of hyphae that formed line of reaction were observed. A compatible reaction was observed only when cultures of the same isolate were paired.
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