Очистка антигена вируса классической чумы свиней для определения антител в иммуноферментном анализе
2009
Garanovich, M.M., National Academy of Sciences. Scientific and Practical Center of Animal Breding (Belarus). The S.N. Vyshelesskij Inst. of Experimental Veterinary Medicine
A method of development of highly purified antigen of a virus of classical swine fever (Sinlak strain) for definition of antibodies in enzyme multiplied immunoassay (ELISA) was tested in the conditions of the Republic of Belarus. There were determined parameters of sensitivity and specificity. There was developed a method of partial purification of the antigens by means of obtaining of infected leukoconcentrate, settling of virus by means of ammonium sulfate with the further ultrafiltration through a membrane
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