Reseach on embryo rescue aiming at the new tea breeding for the high yield and good quality
2010
Nguyen Van Thiep; Nguyen Thi Thu Ha; Trinh Thi Kim My; La Tuan Nghia/
Based on result of identified genetic distance, tea varieties genetic similarily levels from 0.5 to 0.6 as parents of hybrid pairs have been selected. Then sexual hybridization were conducted. The resulted immature embryo were collected for in vitro culture using MS medium added with 2 mg/l of 2.4 D. The highest rate of callus formation from embryo id from 2.56 to 33.21% depending on the time of embryo collection after sexual hybridization. Culture of embryos at 4-5-month age in the MS medium added with 2 mg/l of GA3 resulted in direct development of buds from embryos. The coefficient of rapid propagation of F1 hybrids in vitro, ranged from 3.0 to 6.6 times/one generation . Estimate of the growth of 11 F1 hybrids in the nursery contitions conditions showed that there were tow lines that had highest stem height ; they are TKT-10 (45.6cm high) and PBT 25 (48.0 cm high). Both of them are higher than the local check (Trung Du variety).
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