Optimization of SSR reaction system for identifying the authenticity of maize hybrids | SSR村绫冲绉瀹у搴浣绯荤浼绌
2011
Yue Jing, Shenyang University of Chemical Technology, Liaoning (China)锛Staff Room of Food Science and Engineering | Zhu Zhicheng, Seed Administration Bureau of Liaoning Province, Liaoning(China)
[Objective] The aim was to optimize SSR reaction system applied in the identification of authenticity of maize variety. [Method] The technical parameters of SSR including PCR reaction system, annealing temperature and electrophoresis time were optimized to identify 10 major maize varieties in Liaoning Province. [Result] The optimum PCR reaction system was: 14.60 渭l sterile ultrapure water, 2.00 渭l 10 Buffer (Mg2 +), 1.20 渭l dNTPs, 0.20 渭l Taq enzyme, 0.50 渭l each of the forward and reverse primers and 1.00 渭l DNA stock solution. Annealing temperature and electrophoresis time could greatly influenced the results of PCR amplification. The optimal annealing temperature and electrophoresis time required for the ideal electrophoresis bands under the same conditions were different when different primers were used. [Conclusion] The system is feasible to apply in rapid identification of authenticity of hybrid maize varieties.
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