Detection of multiple fungicide resistance in Botrytis cinerea from tomato in Beijing | 北京地区番茄灰霉病菌的多重抗药性检测

Chino. 摘　　要：2009年12月2010年5月，在北京12个郊区县采集番茄病标样150份，分离纯化得到109个灰葡萄孢（Botrytis cinerea）单孢菌株，用最低抑制浓度法（MIC）测定了其对苯并咪唑类（多菌灵）、二甲酰亚胺类（腐霉利）和氨基甲酸酯类（乙霉威）杀菌剂的抗药性。结果表明：番茄灰霉病菌对多菌灵、腐霉利和乙霉威产生抗性菌株的频率分别为96．3％、80．7％和58．7％；所测菌株对3类杀菌剂的抗性类型有BenRDicSNPCS、BenSDicSNPCR、BenRDicRNPCS和BenRDicRNPCR4种，所占比例分别是19．3％、3．7％、21．1％和56．0％，表明北京地区番茄灰霉病菌对苯并咪唑类、二甲酰亚胺类和氨基甲酸酯类三类杀菌剂的抗药性严重，在生产中需慎用，应选择一些替代的新型杀菌剂和生物农药。

Inglés. From December of 2009 to May of 2010, 150 Botrytis cinerea samples were collected from 12 suburban counties in Beijing, and 109 isolates were purified from them. The isolates were detected of their multiple fungicide resistance to benzimidazole （carbendazim）, dicarboximides （procymidone） and carbamate （diethofencarb） by minimal inhibitory concentration （MIC） method. The results showed that the percentages of carbendazim resist- ance （BenR）, procymidone resistance （DicsR） and diethofencarb resistance （NPCR） were 96.3%, 80.7% and 58. 7%, respectively, and the resistance types were BenRDicSNPCs, BenSDicSNPCR, BenRDicRNPCs and BenRDicRNPCR, accounting for 19.3%, 3.7%, 21.1% and 56.0%, respectively. These results suggested that benzimidazole （carbendazim）, dicarboximides （procymidone） and carbamate （diethofencarb） resistances were common and severe in Beijing. It is necessary to use new types of fungicides or bio-fungicides to control Botrytis cinerea.