Molecular Detection and Differentiation of Mycobacterium tuberculosis Complex in Human Sputum Samples Using PCR Assays: A Preliminary Report.
2009
Thangaselvam, M. | Kidangan, A. | Verma, Rishendra | Ramane, S. P.
Thirty five human sputum collected from TB hospital Bareilly were investigated for Mycobacteria based on direct microscopy, culture and by multiplex peR targeting 12.7 kb fragment and IS 611O. DNA was isolated directly forms putums amples. Outof35 samples,25 were smear positive and 18 yielded culture and 16 were positive by the multiplex PeR. 10 samples were negative on smear mircoscopy, culture and PCR.
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