Haemolytic properties and titration of haemolysins of Staphylococcus aureus of milk origin from cattle and goat with clinical mastitis.
2010
Upadhyay, A. | Kataria, A.K.
Thirty Staphylococcus aureus isolates used in the study obtained from cattle (20) and goat (10) were haemolytic on blood agar. Twenty one of the isolates (14 from cattle, 7 from goats) produced a-haemolysis, 3 produced b-haemolysis (2 from cattle and 1 from goats), and 6 isolates (4 from cattle and 2 from goats) produced both a- and b-haemolysis. The haemolysins tested against erythrocytes from rabbit, cattle and horse in order to demonstrate a-, b- and d-toxins, respectively revealed that a- and b-toxins were produced by all the isolates but b toxin was produced by only 7 isolates from cattle and by 3 from goats. On titration it was recorded that highest titre was recorded for a-toxins (for cattle, 1:2560 and for goat, 1:1280) whereas the highest titres for b and d-toxins was similar (1:160) for cattle as well as goat isolates. The result obtained for qualitative and quantitative haemolysin assays correlated well with the haemolysis pattern seen on the blood agar plates.
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