Supercritical CO₂ for Efficient Extraction of Polymethoxyflavones in Kaempferia parviflora
2011
Wongsrikaew, Natcha, Chung-Ang University, Anseong, Republic of Korea | Woo, H.C., Chung-Ang University, Anseong, Republic of Korea | Vichitphan, Kanit, Khon Kaen University, Khon Kaen, Thailand | Han, J.H., Chung-Ang University, Anseong, Republic of Korea
To ensure polymethoxyflavone (PMF) extract from krachaidum, Kaempferia parviflora rhizome, supercritical CO₂ extraction was attempted as an efficient and nontoxic method. Extraction yields and composition of PMFs in the supercritical fluid extracts (SFEs) were obtained under the different extraction conditions of pressure and temperature. Nine PMFs, including 5,7-dimethoxyflavone (1), 5,7,4'-trimethoxyflavone (2), 5,7,3',4'-tetramethoxyflavone (3), 5-hydroxy-7-methoxyflavone (4), 5-hydroxy-3,7-dimethoxyflavone (5), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (6), 3,5,7-trimethoxyflavone (7), 3,5,7,4'-tetramethoxyflavone (8) and 3,5,7,3',4'-pentamethoxyflavone (9), were isolated by vacuum liquid chromatography and characterized using various spectroscopic methods. The extraction yield by supercritical CO₂ extraction was highest at 60℃ and 300 bar, and the content of PMFs reached up to 85% in the SFEs.
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