Is bleach-sedimented smear microscopy an alternative to direct microscopy under programme conditions in India?
2013
Bhat, P. | Alavadi, U. | Bansal, A. | Desikan, P. | Jaju, J. | Moonan, P.K. | Ohri, B.S. | Rao Shrinivas, M.S. | Rao, V.G. | Satyanarayana, S. | Vishnu, P.H.
This cross-sectional multi-centric study compared the yield of and potential benefit for detecting smear-positive pulmonary tuberculosis (PTB) by bleach sedimentation (2% sodium-hypochlorite) versus direct microscopy under programme conditions in India. Among 3168 PTB suspects, 684 (21.6%) were detected by bleach sedimentation vs. 625 (19.7%) by direct microscopy, with a proportional overall agreement of 96% ( 0.88). While 594 patients were smear-positive with both methods, 31 patients detected by direct microscopy were missed and an additional 90 patients were detected by bleach sedimentation. Overall, bleach sedimentation increased the yield of smear-positive TB detection; however; it also increased the time to results.
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