From the comparative analysis of fungal mitochondrial genes to the development of taxonomic and phylogenetic tools
2011
Barroso , Gérard (INRA , Villenave D'Ornon (France). UR 1264 Mycologie et Sécurité des Aliments) | Ferandon , Cyril (Université de Bordeaux Ségalen (Bordeaux 2), Bordeaux(France).) | Callac , Philippe (INRA , Villenave D'Ornon (France). UR 1264 Mycologie et Sécurité des Aliments)
The complete sequence of the mitochondrial cox1 gene, encoding the largest subunit of the cytochrome oxidase of the Basidiomycota Agaricus bisporus has been achieved. It has the longest cox1 gene (29,902 nt) with the largest number of group I introns (18 group I introns) reported to date in any eukaryote. The group I introns in the A. bisporus cox1 gene are similar to those reported in other Basidiomycetes includeing: 3 of the 4 introns in Agrocybe aegerita, 7 of the 9 introns in Pleurotus ostreatus, 3 of the 6 introns in Moniliophthora perniciosa, and 10 of the 15 introns in Trametes cingulata, constituting 18 of the 23 introns described in all Basidiomycota available genes, Moreover, the A. bisporus cox1 gene possesses two introns specifically reported in this gene ( iAbi1 and iAbi14) and one intron ( iAbi 18) possessing orthologous sequences only in the Ascomycota phylum but unknown to date in the Basidiomycota. Hence, A. bisporus cox1 gene contains three-quarters (18/24) of the fungal introns described in all the fungal cox1 genes from Dikarya (Ascomycota and Basidiomycota). From the A. bisporus sequence, primers were designed to evaluate the potential of rare and widely distributed introns to act as taxonomic and/or phylogenetic markers of species belonging to the genus Agaricus. Indeed, we found that the rare introns could be specifically recovered in some strains and/or species. Sequences of the widely distributed fungal introns provide information on the phylogeny and spread of introns among distant as well as closely related species.
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