Shootlets formation of Eurycoma longifolia through direct regeneration techniques
2001
Sobri, H. | Marziah, M.
Eurycoma longifolia (Tongkat Ali) belongs to the family of the Simaroubaceae. It is a single-stemmed slow-growing understorey tree of the rainforests of Southeast Asia. It is used throughout the region as a herbal medicine and tonic. Chemical compounds such as quassinoids, sitosterol, stigmasterol, melianone, niloticin, scopoletin, eurycomanol, eurylene, hispidone, campesterol and several others have been characterized in leaves, rots and stems of E. longifolia. Most of the active ingredients are concentrated in the taproot of the plant. Tongkat Ali planted at Plant Genetic Center (PGC) in Universiti Putra Malaysia (UPM) was employed in carrying out most of the experiments this study. Some plant meterials from Pulau Langkawi and Hutan Belum Perak, Malaysia were also used. DKW medium supplemented with 1 mg/L and 2 mg/L BAP induce the highest percentage of shoots formation (75) and followed by 3 mg/L BAP (50) and 4 mg/L BAP (25). The same phenomenon is noticed in treatment with WPM. The effect of media composition on the direct regeneration studies show that MS medium is not suitable for higher shoot formation compared to DKW and WPM medium.
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