Strain characterization of selected isolates of sugarcane mosaic virus

Cristobal, D.B.; Dela Cueva, F.M.

Strain characterization of selected isolates of sugarcane mosaic virus

2012

Cristobal, D.B. | Dela Cueva, F.M.

Sugarcane mosaic is one of the most widely distributed diseases of sugarcane caused by four species of Potyvirus. Sugarcane Mosaic Potyvirus (SCMV) has been found to infect a wide range of hosts such as sugarcane and oil palm. Mechanical inoculation of virus-infected sugarcane and oil palm on sorghum was performed. Total RNA was extracted from field collected symptomatic sugarcane and oil palm, and mechanically inoculated sorghum using CTAB and TRizol method. CDN was synthesized using M-MLV RT and amplified using virus-specific primers (SCMV F3, SCMV R3). Amplicon was digested using restriction enzymes Apo1, Hinf1, Taq1, and Xba 1. Preliminary results on strain identification of restriction fragment length polymorphism of amplification products show variation among isolates.

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Palabras clave de AGROVOC

hosts inoculation rflp saccharum officinarum sugarcane mosaic potyvirus

Información bibliográfica

Editorial

PMCP

Paginación

p. 99

Otras materias

Potyvirus mosaique canne a sucre; Huespedes; Inoculacion; Potyvirus mosaico cana de azucar; Hote

Idioma

Inglés

Nota

Summary only

Received 2013

Tipo

Conference; Non-Conventional; Summary

Fuente

PMCP strategies toward better pest management by 2022: proceedings, Pest Management Council of the Philippines, College, Laguna (Philippines).- College, Laguna (Philippines): PMCP, 2012. 43. Pest Management Council of the Philippine Anniversary and Annual Scientific Conference,43. Pest Management Council of the Philippine Anniversary and Annual Scientific Conference, Cagayan De Oro City (Philippines), 8-11 May 2012.- p. 99

Conferencia

43. Pest Management Council of the Philippine Anniversary and Annual Scientific Conference, , Cagayan De Oro City (Philippines), 8-11 May 2012

En AGRIS desde: 2014-07-15

Formato: AGRIS AP

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