In vitro propagation and multiple shoot induction of Rhodiola rosea L. by axillary bud culture
2012
Bae, K.H., Hongcheon Institute of Medicinal Herb, Hongcheon, Republic of Korea | Ko, M.S., Jeju Biodiversity Research Institute, Jeju Technopark, Seogwipo, Republic of Korea | Kim, N.Y., Korea National Arboretum, Pocheon, Republic of Korea | Song, J.M., Nature Environment Research Office of Gangwon Province, Hongcheon, Republic of Korea | Song, G.P., Jeju Environment Research Institute, Jeju, Republic of Korea
An efficient in vitro propagation was established by using axillary bud explants of roseroot (Rhodiola rosea L.), which has been known as a medicinal plant in East Asia. Among various media tested, MS medium supplemented with 1.0 mg/L BA and 1.0 mg/L GA₃ was found to be the best for multiple shoot formation (15 axillary shoots per axillary bud). In addition 1/2MS medium containing 50 g/L sucrose was best for shoot elongation (7.8 cm) and increasing total chlorophyll contents (8.64 mg/g) best. Maximum number of roots (17.7 roots per explant) was observed on the medium without plant growth regulators. Propagated plants were successfully acclimatized to ex vitro conditions, with a survival frequency of 97% after 12 weeks. Most rooted shoots grew well and produced viable seeds when grown in vitro culture conditions. Therefore, R. rosea can be effectively propagated in vitro by the system we developed in this study.
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