Effects of Supercritical Fluid Extract, Shikonin and Acetylshikonin from Lithospermum erythrorhizon on Chondrocytes and MIA-Induced Osteoarthiritis in Rats
2013
Kim, G.S., RDA, Eumseon, Republic of Korea | Kim, H.J., YD Life Science Co. Ltd., Seoul, Republic of Korea | Lee, D.Y., RDA, Eumseon, Republic of Korea | Choi, S.M., YD Life Science Co. Ltd., Seoul, Republic of Korea | Lee, S.E., RDA, Eumseon, Republic of Korea | Noh, H.J., RDA, Eumseon, Republic of Korea | Choi, J.G., YD Life Science Co. Ltd., Seoul, Republic of Korea | Choi, S.I., YD Life Science Co. Ltd., Seoul, Republic of Korea
This study investigates the effect of supercritical fluid extract(CMPB803-C) of Lithospermum erythrorhizon, shikonin and acetylshikonin isolated from Lithospermum erythrohizon on IL-1β-induced chondrocytes and monosodium iodoacetate(MIA)-induced osteoarthritis in rat. Shikonin(50mM) and acetylshikonin( 3mM) treatment reduced significantly the mRNA expression and enzyme avtivity of matrix metalloproteinase (MMP) -1,-3 and -13 in IL-1β-induced SW1353 chondrosarcoma cells. The chondro-protectie effects of CMPB803-C and acetylshikonin were than analyzed in a rat OA model using a single intra-articular injection of MIA(1mg) in the right knee joint.CMpB803-C(200mg/kg) or acetylshikonin (5mg/kg) was orally administered daily for two weeks starting after 1 week of MIA injection . In the histological observation , CMPB803-C and acetylshikonin clearly improved OA lesions being comparable to or better that control group. Our results demonstrated that CMPB803-C and acetylshikonin as active compound of Lithospermum erythrorhizon have a strong chondro-protective effect in OA rats, which likely attributes to its anti-inflammatory activity and inhibition of MMPs production.
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