Antioxidant and Antiaging Assays of Hibiscus sabdariffa Extract and Its Compounds

Skin aging is a complex biological process due to intrinsic and extrinsic factors. Free radical oxidative is one of extrinsic factors that induce activation of collagenase, elastase and hyaluronidase. Natural product from plants has been used as antioxidant and antiaging. This study aimed to evaluate antioxidant and antiaging properties of Hibiscus sabdariffa extract (HSE) and its compounds including myricetin, ascorbic acid, and beta carotene. The phytochemical of H. sabdariffa was determined using modified Farnsworth method and presence of phenols, flavonoids and tannins were in moderate content, whereas triterpenoids and alkaloids were in low content. Total phenolic content performed using Folin-Ciocalteu method, was 23.85 microgram GAE/mg. Quantitative analysis of myricetin, beta-carotene, and ascorbic acid of HSE was performed with Ultra-High Performance Liquid Chromatography (UHPLC) that shows 78.23 microgram/mg myricetin, 0.034 ㎍/mg beta-carotene, whilst ascorbic acid was not detected. HSE has lower activity on DPPH (IC50 = 195.73 microgram/mL) compared to beta-carotene, the lowest in ABTS assay (IC50 = 74.58 microgram/mL) and low activity in FRAP assay (46.24 micrometer Fe(II)/microgram) compared to myricetin, beta-carotene. Antiaging was measured through inhibitory activity of collagenase, elastase, and hyaluronidase. HSE had weakest collagenase inhibitory activity (IC50= 750.33 microgram/mL), elastase inhibitory activity (103.83 microgram/mL), hyaluronidase inhibitory activity (IC50 = 619.43 microgram/mL) compared to myricetin, beta-carotene, and ascorbic acid. HSE contain higher myricetin compared to beta-carotene. HSE has moderate antioxidants and lowest antiaging activities. Myricetin is the most active both antioxidant and antiaging activities.