Molecular screening of aroma genes in indigenous and exotic rice germplasm
Ashfaq, M. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Farooq, H.U. (Punjab Univ., Lahore (Pakistan). Inst. of Agricultural Sciences) | Sabar, M. (Rice Research Inst., Kala Shah Kaku (Pakistan)) | Ali, A. | Ali, M.
Aromatic rice varieties are famous all over the world for its specific taste, superfine long grains and specific aroma which is caused by 2-acetyl-1- Pyroline (2AP) compound. Molecular markers provide the easiest way to distinguish aromatic and non-aromatic varieties on the basis of polymorphism expressed in specific band sizes. A mutation resulting from 8 by deletions on exon 7 in a gene (badh2) which encodes betaine aldehyde dehydrogenase of chromosome 8 is responsible for the aromatic expression. Two external primers produce a band of 580 by which is a control check for both aromatic and non-aromatic lines. Two Internal primers anneal with their respective external primers and give 257 by size band for aromatic and 355 by for non-aromatic rice lines. Using gene specific marker for aroma, 75 genotypes of rice were screened. PCR analysis showed that 28 varieties were aromatic, 6 varieties were heterozygous and 41 varieties were nonaromatic. These results will be helpful for the rice breeders to develop new aromatic rice varieties by exploiting the identified rice genotypes carrying aroma gene.
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