Physiological, antioxidant characteristics and proximate composition of radish leaves

The present research has been designed to investigate the effect of foliar application of different plant extract as a source of natural growth regulators on nutritional attributes of radish. Seeds of radish were sprouted in plastics pots placed in a greenhouse. Experiment was established in a sandy loam soil with urea. After seed germination foliar spray of Moringa leaf extract (MLE), Mulberry leaf extract (MuLE), Sorghum leaf extract (SLE) and Brassica leaf extract (BLE) at three different concentrations (3%, 5% and 10%) were applied at the rate of 160 L/Hactare. The first spray was applied after 25-30 Days After Emergence (DAE) and later, the second and third sprays were made at an interval of two weeks. The study used a negative control (without exogenous application) and a positive control (water @ 160 L/Hectare) for comparison. The leaf samples were collected at peak vegetative stage and subjected to different groups of analysis. The observed values for percentage yield of Raphanus sativus. L were 3.2-20.5%, total phenolic contents (TPC) of radish ranged from 29.76 to 51.06 (GAE mg/100gm of dry weight) and it was found that 3% concentration applying in all the treatments gave higher values. DPPH free radical scavenging capacity (IC50) value for radish ranged from 0.98-1.97 mug/ml. Reducing power in the range of 0.14-0.82 of the concentration of 10 mg/g. Chlorophyll a and chlorophyll b contents in the range of 0.40 - 0.79 and 0.30 -1.47, Proline contents were found in the range of 0.604-3.65. Moisture contents and ash contents were found in the range of 13.7- 47.2 and 13.8-18.8, respectively. The physiological parameters such as number of leaves, leaf area, average height and leaf weight were found in the range of 6-10, 11-13, 8-10 and 3.49-6.87 respectively. Hence it was observed that all the applied PGRs have more value as compared to water control and U-controI. Moringa with 3% concentration showed the best performance among all the applied PGRs.