Medfly microsatellite markers for species diagnosis and population genetic analysis in three other fruit fly (Diptera : Tephritidae) species
2002
Baliraine, F.N. | Bonizzoni, M. | Osir, E.O. | Lux, Slawomir A. | Mulaa, F.J. | Quilici, Serge | Gomulski, L.M. | Gasperi, Giuliano | Malacrida, Anna R.
Tephritid fruit flies of the genus Ceratitis MacLeay (Dacinae: Ceratitidini) pose one of the greatest threats to Africa's horticultural industry. To curb their economic injury and prevent spread to new areas, it is vital that quarantine workers and developers of control tools and strategies can unambiguously identify the different species atany life age. However, the classification ofTephritidae to the species level based on morphology alone continues to be difficult. We have sought to utilize molecular markers, which are life-stage independent, stable and intrinsic to the organisms, for this purpose. Consequently, we screened 24 Medfly (Ceratitis capitata) microsatellite markers for cross-species amplification in C. rosa, C. fasciventris and C. cosyra. The presence or absence of each locus in each species was tested by PCR, and negative results confirmed by Southern analysis. Locus homology species was tested by PCR, and negative result confirmed by sequencing. Our findings indicate that is is possible to distinguish the different species examine using a combination of the medfly-bases primers. Of the 24 loci screene, 17 were common to all, five were absent in C. Cosyra, three were absent in C. Rosa, two were absent in C. Fasciventris, while on locus was present only in C. capitata. In addition, sequence results indicate that most of the medfly-based microsatellite markers could be useful for population genetic studies in the species tested, an aspect wich would facilitate the tracing of the geographical origin of colonist pest populations, assessment of their invasive potential and risk assessment. (Résumé d'auteur)
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