Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity
2019
Giraud, Sébastien | Steichen, Clara | Couturier, Pierre | Tillet, Solenne | Mallet, Vanessa | Coudroy, Rémi | Goujon, Jean-Michel | Hannaert, Patrick | Hauet, Thierry
Ischemia-reperfusion (IR) injury is unavoidable during organ transplantation and impacts graft quality. New paradigms areemerging including preservation at higher temperature than “hypothermia” or “cold”: although 4∘Cremainslargelyusedforkidney preservation, recent studies challenged this choice. We and others hypothesized that a higher preservation temperature,closer to physiological regimen, could improve organ quality. For this purpose, we used anin vitromodel of endothelial cellsexposed to hypoxia-reoxygenation sequence (mimicking IR) and anex vivoischemic pig kidneys static storage model.In vitro,19∘C, 27∘C, and 32∘C provided protection against injuries versus 4∘C, by reducing cell death, mitochondrial dysfunction, leukocyteadhesion, and inflammation. However,ex vivo, the benefits of 19∘Cor32∘C were limited, showing similar levels of tissue preservationdamage.Ex vivo4∘C-preserved kidneys displayed a trend towards reduced damage, including apoptosis. Macrophage infiltration,tubulitis, and necrosis were increased in the 19∘Cand32∘Cversus4∘C preserved kidneys. Thus, despite a trend for an advantageof subnormothermia as preservation temperature, ourin vitroandex vivomodels bring different insights in terms of preservationtemperature effect. This study suggests that temperature optimization for kidney preservation will require thorough investigation,combining the use of complementary relevant models and the design of elaborated preservation solution and new technologies.
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