Haemophilus paragallinarum haemagglutinin: Role in adhesion, serotyping and pathogenicity
2008
Barnard, T.G.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Van Heerden, E.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Bragg, R.R.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology) | Albertyn, J.(University of the Free State Faculty of Natural and Agricultural Science Department of Microbial, Biochemical and Food Biotechnology)
It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pi and molecular mass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 °C. It was also shown that activity is enhanced by the addition of Ca²+ and Mn²+ ions.
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