Callus proliferation in woody plant of Michelia Alba dc. young flower and leaf explants
2003
Nor'aishah A.S. | Radzali M.
Two types of established basal media were used in order to select the best media for callus proliferation and organogenesis in Michelia alba flower and leaf explants. Petal excised from flower of stage 8 and lamina with midrib from leaf of stage 2 was separately cultured on MS (1962) medium and woody plant medium (Lloyd and McCown 1981). Each of the studied media was supplemented with various concentrations of plant growth regulators such as NAA and BAP. Both media with supplemented with plant growth regulators have produced pale colour and friable callus. However, the woody plant media produce remarkable callus proliferation compare to MS medium which are around 50% to 100% of the explants have induced callus formation. Most of the calluses were friable to compactly nodular and leaf explants produce yield than flower explants.
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