IMPACT OF OOCYTE SIZE ON LHRHa INDUCED OVULATION AND FERTILIZED EGG QUALITY IN SADDLED BREAM OBLADA MELANURA (LINNAEUS, 1758)
2013
Nenad Antolović | Valter Kožul | Nikša Glavić | Jakša Bolotin
The objective of this study was to evaluate the effects of oocyte size and luteinizing hormone releasing hormone analogue (LHRHa) on ovulatory success in artificial fertilization. Vitellogenic females with maximum oocyte diameters 400-550 µm were repeatedly injected with LHRHa (20 µg kg-1 per injection). Fish with maximum oocyte diameters <500 µm failed to ovulate. In contrast, all fish with maximum oocyte diameters >500 µm spawned within 48-54 h. These results demonstrate that injected LHRHa is effective for ovulation of saddled bream with maximum oocyte diameters >500µm.
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