Comparative Proteomics Analysis of Phosphine-Resistant and Phosphine-Susceptible Sitophilus oryzae (Coleoptera: Curculionidae)
2021
Hyun-Na Koo | Seung Ju Seok | Hyun Kyung Kim | Gil-Hah Kim | Jeong Oh Yang
A proteomic method combining two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF) was used to compare the protein expression profiles of phosphine-resistant (PH<sub>3</sub>-R) and -susceptible (PH<sub>3</sub>-S) strains of Sitophilus oryzae. Thirty-nine differentially expressed protein spots were identified between the PH<sub>3</sub>-R and PH<sub>3</sub>-S strains; 20 protein spots were upregulated, and 19 protein spots were downregulated in the PH<sub>3</sub>-R strain compared with their expression in the PH<sub>3</sub>-S strain. In particular, cytochrome oxidase subunit I showed 15-fold higher expression in the PH<sub>3</sub>-R strain than in the PH<sub>3</sub>-S strain. Additionally, citrate synthase 2, delta-1-pyrolline-5-carboxylate dehydrogenase, and triose-phosphate isomerase were highly expressed in the PH<sub>3</sub>-R strain. In summary, our study has improved understanding of the molecular mechanisms of phosphine resistance in the rice weevil.
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