QTL identification for tolerance to fruit set in tomato by fAFLP markers
2007
Gisele Ventura Garcia Grilli | Leila Trevizan Braz | Eliana Gertrudes Macedo Lemos
This study aimed to detect quantitative trait loci (QTL) by fAFLP (Fluorescent Amplified Fragment LengthPolymorphism) markers associated to the trait tomato fruit set at high temperatures. A biparental cross between line Jab-95(heat-tolerant) and cultivar Caribe (heat-susceptible) was made. A total of 192 plants of the F2 generation were evaluated,generating 172 polymorphic markers through six primer combinations previously identified by the Bulked Segregant Analysistechnique. To construct the genetic map, 106 of the 172 markers that segregated in the expected Mendelian segregationproportion (3:1) were used. The map covered 1191.46 cM of the genome. Six trait-linked QTL were identified in the analysisof simple markers and three others by the interval-mapping methodology. These results could be highly useful in improvementprograms, since heat-tolerant plants can be selected rapidly, which improves tomato fruit set.
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