PDX1.1-dependent biosynthesis of vitamin B6 protects roots from ammonium-induced oxidative stress
2022
Liu, Ying | Maniero, Rodolfo, | Giehl, Ricardo F.H. | Melzer, Michael | Steensma, Priscille | Krouk, Gabriel | Fitzpatrick, Teresa, | von Wirén, Nicolaus
Despite serving as a major inorganic nitrogen source for plants, ammonium causes toxicity at elevated concentrations, inhibiting root elongation early on. While previous studies have shown that ammonium-inhibited root development relates to ammonium uptake and formation of reactive oxygen species (ROS) in roots, it remained open which mechanisms are underlying the repression of root growth and how plants cope with this inhibitory effect of ammonium. Here, we demonstrate that ammonium-induced apoplastic acidification co-localizes with Fe precipitation and hydrogen peroxide (H2O2) accumulation along the stele of the elongation and differentiation zone in root tips, indicating Fe-dependent ROS formation. By screening ammonium sensitivity in T-DNA insertion lines of ammonium-responsive genes, we identified PDX1.1, which is upregulated by ammonium in the root stele and catalyzes biosynthesis de novo of vitamin B6. Root growth of pdx1.1 mutants is hypersensitive to ammonium, while chemical complementation or overexpression of PDX1.1 restores root elongation. This salvage strategy requires non-phosphorylated forms of vitamin B6 that are able to quench reactive molecular oxygen species and rescue root growth from ammonium inhibition. We propose PDX1.1-mediated synthesis of non-phosphorylated B6 vitamers as a primary strategy to protect roots from ammonium-dependent ROS formation.
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