Differentiation of biologically distinct Cucumber mosaic isolates by double stranded RNA profile
2002
and B.K. GIRl, SHELLY PRAVEEN, SAVARNI TRlPATHI
Four isolates of Cucumber mosaic virus from Nicotiana tabacum, Commellina species and Petunia, designated as MV- T, C, PI and P 2 , differed in their host reaction, temperature sensitivity, and at the molecular level. Serologically related isolates of CMV viz. CMV-P I , P 2 , C and T were analyzed on the basis of their symptomology, temperature tolerance and double stranded RNA pattern. Double stranded RNA (ds-RNA) pattern of these CMV isolates representing biological diversity were examined. Two distinct patterns differing by the number and or/position of the ds- RNA bands were obtained for the four isolates. The ds-RNA pattern can not be necessarily co-related with the symptoms severity nor with any specific biological characteristics, however, it can be utilized in distinguishing isolates.
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