Bacillus megaterium from tea rhizosphere promotes growth and induces systemic resistance in tea against Sclerotium rolfsii
2015
U. CHAKRABORTY*, A.P. CHAKRABORTY, B.N. CHAKRABORTY and
The objectives of the study was to determine how Bacillus megaterium, isolated from tea rhizosphere promotes the growth of tea plants as plant growth promoter, affects sclerotial blight disease and induces systemic resistance in tea plants. Bacillus megaterium, promoted growth of 5 varieties of tea, as evidenced by increase in height, emergence of new leaves, branches and increase in leaf numbers both in potted conditions and in the field. The bacterium was antagonistic to a number of fungal pathogens in vitro and reduced sclerotial blight of tea caused by Sclerotium rolfsii. Significant increases in phenolics along with increase in isomers of catechins as well as peroxidase, chitinase, β-1,3-glucanase and phenylalanine ammonia-lyase, were observed in tea plants on application of B. megaterium alone or following challenge inoculation with S. rolfsii. Survival of B. megaterium in soil was determined by ELISA and Dot-Blot using PAb raised against the bacterium. Enhanced expression of chitinase in B. megaterium treated leaves was confirmed by Western Blot and Dot Blot using PAb raised against chitinase. Besides, immunolocalization of chitinase in tea leaves was also done using the chitinase PAb. B. megaterium has been found to be a good plant growth promoter with the ability to increase growth of tea plants along with a reduction of sclerotial blight disease which acts both by direct and indirect mechanisms in the host.
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