Pathogenicity and immunohistochemical detection of infectious bursal disease virus in specific pathogen-free chickens
1999
Mahani A. H. | Sharifah S. H. | Zuraidah A. | Kamil W. M.
Only two out of eight infectious bursal disease (IBD)virus isolated from the field were capable of causing 100% mortality of 4 week-old specific pathogen free chickens within 5 days post-inoculation (pi). Typical gross and histological IBD lesions were seen in all lymphoid organs of chickens that died within 5days pi. Chickens that were culled at 6 and 10 days pi showed only atrophy in the bursa of Fabricius (BF). Immunoperoxidase staining revealed positive reactions in the tissues of the BF, spleen, thymus and caecal tonsil of chickens that died within 5 days pi and in the BF of chickens culled within 10 days pi.
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