Glycation and Oxidative Stress Increase Autoantibodies in the Elderly
2020
Mohd W.A. Khan | Ahmed Al Otaibi | Subuhi Sherwani | Wahid A. Khan | Eida M. Alshammari | Salma A. Al-Zahrani | Mohd Saleem | Shahper N. Khan | Sultan Alouffi
Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance in the elderly. This study aims to understand links among aging, gluco-oxidative stress, and autoantibodies in asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemical changes. Significant inhibition of advanced glycation end products (AGEs) formation was achieved using garlic extract (53.75%) and epigallocatechin-3-gallate from green tea (72.5%). Increased amounts of serum carbonyl content (2.42 ±: 0.5) and pentosidine (0.0321 ±: 0.0029) were detected in IV-S (S represent smokers) vs. IV group individuals. Direct binding ELISA results exhibited significantly high autoantibodies against Gly-HSA in group IV-S (0.55 ±: 0.054: p <: 0.001) and III-S (0.40 ±: 0.044: p <: 0.01) individuals as compared to the age matched subjects who were non-smokers (group IV and III). Moreover, high average percent inhibition (51.3 ±: 4.1%) was obtained against Gly-HSA in IV-S group individuals. Apparent association constant was found to be high for serum immunoglobulin-G (IgG) from group IV-S (1.18 ×: 10&minus:6 M) vs. serum IgG from IV group (3.32 ×: 10&minus:7 M). Aging induced gluco-oxidative stress and AGEs formation may generate neo-epitopes on blood-proteins, contributing to production of autoantibodies in the elderly, especially smokers. Use of anti-glycation natural products may reduce age-related pathophysiological changes.
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