Mutation frequency in mungo (Phaseolus radiatus L.) after treatment with an alkylating chemical mutagen [study conducted in the Philippines]
1980
Palima, C.J. | Soriano, J.D.
Dormant seeds of green mungo (Phaseolus radiatus L.) were treated with aqueous solution of EMS at concentrations of 0.02M to 0.06 M with phosphate buffer for periods of 6 to 24 hours at approximately 30 C. Seeds soaked in distilled water and buffer solution were used as controls. The seeds were grown on moist blotting paper for germination percentage and seedling height measurements after seven days. The M1 seedling were transplanted in field plots in a dose-to row plant with two replications for determination of the frequency of somatic mutations, percentage of field survival at flowering stage, and seed-set. The M2 progenies were grown for determination of types and frequency of chlorophyll-deficient mutations as an index of the degree of genetic change due to the mutagen. The effectivity of the mutagen are measured by using percentage of germination after treatment, seedling height, somatic mutations, seed-set and germinal mutation as parameters. Three types of induced chemical sectors on leaves of M1 plants such as pale green, yellow green and yellow occurred with a frequency ranging from 4.65 to 31.03 percent. Four types of chlorophyll-deficient seedling mutations were obtained, i.e., virescent, chlorina, xantha and albina with a frequency ranging from 0.23 to 7.55 percent on the seedling basis and 2 to 26 percent on the pod basis.
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