Evaluation of some commercial pectinases
1987
Al-Bakir, A.Y. | Al-Tai, W.F. | Ali, M.A.A. (Scientific Research Council, Baghdad (Iraq). Agriculture and Water Resources Research Centre, P.O. Box 2416)
An evaluation procedure was developed for some commercial pectinases. The procedure included assay of pectolytic and non-pectolytic activities, determination of protein, total carbohydrates and reducing sugars, and fractionation with affinity chromatography. Ultrazym 100, Rapidase and pectinase contained 132.86, 3.57 and 138.72 polygalacturonase units/mg protein, respectively. It was found that Ultrazym 100 and Rapidase possesed cellulase, amylase, invertase and Beta-galactosidase activity while pectinase contained the first three activities only. The protein content of the three preparations was in the range of 1.6 - 22.5%. Total carbohydrates content of pectinase, Ultrazym 100 and Rapidase solutions (5 mg/ml) was 66, 76 and 36%, respectively. Separation of exo-and endo-polygalacturonases (present in Ultrazym 100) was achieved by affinity chromatography using cross-linked sodium pectate. It was further confirmed by analysis of end products
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