Determination of soil microbial biomass. A survey of methods
1992
Simonovicova, A. (Slovenska Akademia Vied, Bratislava (Slovak Republic). Ustav Ekobiologie)
A review of methods used for the determination of soil microbial biomass is given. The indirect methods are based on counting of microbial colonies developed on various cultivation media. Applying microscopical methods, length and thickness of the hyphae of micromycetes was measured, and bacterial cells in the range of the microscope view were counted. The chemical method indicates the biomass defining ATP - as the cell component. The essence of physiological or biochemical methods is based on adding to the soil such compound, which would affect the microbial processes in the soil sample. The course and the changes of these processes were then observed. The results obtained when using different methods, are comparable after calculation for identical units of each soil sample, e.g. 10 g C.
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